Vertical Laminar Flow Hood Aseptic Technique

The direction of air flow may be horizontal or vertical.

Vertical laminar flow hood aseptic technique.

Vertical laminar flow hoods are often chosen because they resemble on a small scale the design of a laminar flow cleanroom in which fan filter units are typically positioned in the ceiling. Manipulative technique is also different in a vertical flow hood. 99 99 particle free air is forced downward toward the work surface. Room air in red enters the system from above the hepa filter.

What is the maximum number of particles in a clean room buffer room and ante room. T f using a laminar flow hood in an open room is no safer than preparing products on a countertop. Use strict aseptic technique to avoid introduction of contaminants into the hood. Horizontal flow hoods are most commonly used with the more costly vertical flow hoods being reserved for agents that may produce an environmental hazard e g.

Laminar flow hoods are used to control airborne contamination of sterile products during their extemporaneous preparation. While laminar airflow hoods prevent airborne contamination they do not guarantee a sterile product. Supplies may be arranged to the sides and the back of the area in which manipulations will be performed. Development maintenance of good aseptic technique in personnel who prepare administer sterile products 2.

Development maintenance of skills needed to properly use laminar airflow workbench lafw or laminar airflow hood. Cytotoxic agents radioactive agents antimicrobial agents. Development maintenance of sterile compounding area complete with sterilized equipment supplies 3. Turbulence patterns around objects in the vertical flow hood will be different from that in horizontal flow units.

Use precautions to minimize contamination in the product preparation area. The air flowing out from the hood suspends and removes contaminants introduced into the work area by personnel. Items not sterile including fingers and hands must be kept.